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High-frequency plant regeneration via adventitious shoot formation from deembryonated cotyledon explants of Sesamum indicum L

Authors :
M. Y. Choi
H. Y. Seo
Haeng-Ran Kim
Yong Seok Lee
Song-Joong Yun
Yongwon Choi
Y. J. Kim
T. I. Park
K. H. Park
M. K. Oh
C. H. Paik
Source :
In Vitro Cellular & Developmental Biology - Plant. 43:209-214
Publication Year :
2007
Publisher :
Springer Science and Business Media LLC, 2007.

Abstract

Sesamum indicum L. was used as an important oil crop in the world. An efficient protocol for in vitro plant regeneration via adventitious shoot formation from deem-bryonated cotyledon explants isolated from mature seeds of sesame is developed. Optimal medium for direct adventitious shoot formation was Murashige and Skoog (MS) medium with 22.2 μM 6-benzylaminopurin (BA) and 5.7 μM indole-3-acetic acid (IAA). Abscisic acid (3.8 μM ABA) and AgNO3 (29.4 μM) were effective in enhancing the frequency of adventitious shoot formation. Preculture of cotyledon explants on high sucrose concentration (6–9%) for 2 wk and subsequent transfer to 3% sucrose enhanced the frequency of adventitious shoot induction. Root formation from the adventitious shoots was easily achieved on MS medium containing 2.7 μM of α-naphthalene acetic acid (NAA). Regenerated plantlets were acclimatized on sand and peat moss (1:1), showing 95% survival with subsequent flowering and seed set. We established the high-frequency plant rege...

Details

ISSN :
14752689 and 10545476
Volume :
43
Database :
OpenAIRE
Journal :
In Vitro Cellular & Developmental Biology - Plant
Accession number :
edsair.doi...........0dc0f4de5682b0d8c1343e5a3abf095d
Full Text :
https://doi.org/10.1007/s11627-006-9017-2