Back to Search Start Over

[Untitled]

Authors :
Kristen R. Ross
Alf Giese
Jun S. Wei
Jeffrey M. Trent
Michael E. Berens
Dominique B. Hoelzinger
Stephen W. Coons
Theresa J Berens
George S. Watts
Tim Demuth
Luigi Mariani
Wendy S. McDonough
Christian Beaudry
Source :
Journal of Neuro-Oncology. 53:161-176
Publication Year :
2001
Publisher :
Springer Science and Business Media LLC, 2001.

Abstract

Microarray analysis of complementary DNA (cDNA) allows large-scale, comparative, gene expression profiling of two different cell populations. This approach has the potential for elucidating the primary transcription events and genetic cascades responsible for increased glioma cell motility in vitro and invasion in vivo. These genetic determinants could become therapeutic targets. We compared cDNA populations of a glioma cell line (G112) exposed or not to a motility-inducing substrate of cell-derived extracellular matrix (ECM) proteins using two sets of cDNA microarrays of 5,700 and 7,000 gene sequences. The data were analyzed considering the level and consistency of differential expression (outliers) and whether genes involved in pathways of motility, apoptosis, and proliferation were differentially expressed when the motility behavior was engaged. Validation of differential expression of selected genes was performed on additional cell lines and human glioblastoma tissue using quantitative RT-PCR. Some genes involved in cell motility, like tenascin C, neuropilin 2, GAP43, PARG1 (an inhibitor of Rho), PLCy, and CD44, were over expressed; other genes, like adducin 3y and integrins, were down regulated in migrating cells. Many key cell cycle components, like cyclin A and B, and proliferation markers, like PCNA, were strongly down regulated on ECM. Interestingly, genes involved in apoptotic cascades, like Bcl-2 and effector caspases, were differentially expressed, suggesting the global down regulation of proapoptotic components in cells exposed to cell-derived ECM. Overall, our findings indicate a reduced proliferative and apoptotic activity of migrating cells. cDNA microarray analysis has the potential for uncovering genes linking the phenotypic aspects of motility, proliferation, and apoptosis.

Details

ISSN :
0167594X
Volume :
53
Database :
OpenAIRE
Journal :
Journal of Neuro-Oncology
Accession number :
edsair.doi...........0dcf3fb69e468337593d924ecd1a8852
Full Text :
https://doi.org/10.1023/a:1012253317934