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The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury
- Source :
- Open Journal of Anesthesiology. :35-41
- Publication Year :
- 2017
- Publisher :
- Scientific Research Publishing, Inc., 2017.
-
Abstract
- A severe injury can trigger an inflammation response and excessive response can cause multiple organ failure. HMGB1 is an early inflammation mediator in sterile injury and a late inflammation mediator in infection. It is an important mediator in severe sepsis and always associated with the severity of organ failure. Previous studies showed that the administration of systemic lidocaine could inhibit the expression of HMGB1 on septic mice with musculoskeletal injury. Nine male adult Balb/c mice were grouped by simple random sampling method into three groups of intravenous lidocaine injection dosages: 2 mg/kg, 3 mg/kg, 4 mg/kg. Musculoskeletal injury was done by breaking the left femoral bone in a close manner. Peripheral blood sampling was done 4 hours after injury and 2 hours after lidocaine therap. To evaluate the expression level of HMGB1 mRNA, RT-PCR was used. The result of our study showed that intravenous lidoaine administration on the 3 groups could decrease the level of HMGB1. In conclusion, lidocaine hold an important role in clinical diseases by inhibiting HMGB1.
- Subjects :
- 0301 basic medicine
Messenger RNA
Severe injury
Dose
biology
Lidocaine
business.industry
chemical and pharmacologic phenomena
Inflammation
medicine.disease
HMGB1
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Mediator
Anesthesia
Musculoskeletal injury
medicine
biology.protein
medicine.symptom
business
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 21645558 and 21645531
- Database :
- OpenAIRE
- Journal :
- Open Journal of Anesthesiology
- Accession number :
- edsair.doi...........0e55c19b242304050b1332e7c4d048bd
- Full Text :
- https://doi.org/10.4236/ojanes.2017.72004