Back to Search Start Over

The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury

Authors :
Andi Asadul Islam
Robert Hotman Sirait
Mochammad Hatta
Bambang Suprayogi
Tigor Peniel Simanjuntak
Muhammad Ramli
Syafrie Kamsul Arief
Source :
Open Journal of Anesthesiology. :35-41
Publication Year :
2017
Publisher :
Scientific Research Publishing, Inc., 2017.

Abstract

A severe injury can trigger an inflammation response and excessive response can cause multiple organ failure. HMGB1 is an early inflammation mediator in sterile injury and a late inflammation mediator in infection. It is an important mediator in severe sepsis and always associated with the severity of organ failure. Previous studies showed that the administration of systemic lidocaine could inhibit the expression of HMGB1 on septic mice with musculoskeletal injury. Nine male adult Balb/c mice were grouped by simple random sampling method into three groups of intravenous lidocaine injection dosages: 2 mg/kg, 3 mg/kg, 4 mg/kg. Musculoskeletal injury was done by breaking the left femoral bone in a close manner. Peripheral blood sampling was done 4 hours after injury and 2 hours after lidocaine therap. To evaluate the expression level of HMGB1 mRNA, RT-PCR was used. The result of our study showed that intravenous lidoaine administration on the 3 groups could decrease the level of HMGB1. In conclusion, lidocaine hold an important role in clinical diseases by inhibiting HMGB1.

Details

ISSN :
21645558 and 21645531
Database :
OpenAIRE
Journal :
Open Journal of Anesthesiology
Accession number :
edsair.doi...........0e55c19b242304050b1332e7c4d048bd
Full Text :
https://doi.org/10.4236/ojanes.2017.72004