A New Rapid Method for Clostridium difficile DNA Extraction and Detection in Stool

Authors :: Peter C. Iwen
Christine S. Booth
Hendrik J. Viljoen
Alison G. Freifeld
Xing Zhao
Teresa Karre
Scott E. Whitney
Kari A. Simonsen
Source :: The Journal of Molecular Diagnostics. 14:274-279
Publication Year :: 2012
Publisher :: Elsevier BV, 2012.
Abstract: We describe a new method for the rapid diagnosis of Clostridium difficile infection, with stool sample preparation and DNA extraction by heat and physical disruption in a single-use lysis microreactor (LMR), followed by a rapid PCR amplification step. All steps can be accomplished in

Subjects :: Clostridium difficile DNA
Lysis
Extraction (chemistry)
Diagnostic test
Biology
Clostridium difficile
DNA extraction
Pathology and Forensic Medicine
law.invention
Microbiology
law
Molecular Medicine
Polymerase chain reaction
Point of care

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