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Spectrophotometry can monitor changes in algal metabolism triggered by nutrient deficiency in Nannochloropsis oculata cultured under various light-emitting diode light regimes
- Source :
- Fisheries Science. 85:167-176
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Efficient production of beneficial metabolites by microalgae using light-emitting diodes (LED) is attracting more interest. In batch cultures, metabolic changes that occur with nutrient deficiency over time must be monitored to estimate the metabolite content. Our previous study showed that nutrient deficiency in Nannochloropsis oculata can be briefly evaluated by measuring the absorption ratio at 490 and 680 nm (Abs490/Abs680). However, little is known about the relation with this ratio and metabolic change, particularly in LED light-conditioned cultures. In this study, we investigate if Abs490/Abs680 can be an indicator of the biochemical status of N. oculata cultured under various LED color regimes. Three LED treatments were used: Blue (B), Blue + Red (BR), and Red (R). In all LED treatments, the Abs490/Abs680 decreased for several days and then increased when phosphate was limited or non-existent. The trends in the C16:0 and C20:5ɷ3 proportions and chlorophyll a content commonly changed at the turning points of the Abs490/Abs680. Furthermore, significant correlations were found among the metabolic state and absorption ratio, except in treatment R. We suggest that spectrophotometry can be used for monitoring metabolic changes in N. oculata cultures under various LED irradiance, unless there is excessive 630 nm-red light exposure.
- Subjects :
- 0106 biological sciences
Chlorophyll a
medicine.diagnostic_test
010604 marine biology & hydrobiology
Metabolite
04 agricultural and veterinary sciences
Metabolism
Aquatic Science
Phosphate
01 natural sciences
law.invention
chemistry.chemical_compound
chemistry
law
Spectrophotometry
040102 fisheries
medicine
0401 agriculture, forestry, and fisheries
Nannochloropsis oculata
Food science
Nutrient deficiency
Light-emitting diode
Subjects
Details
- ISSN :
- 14442906 and 09199268
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Fisheries Science
- Accession number :
- edsair.doi...........0ece66e654927bcd281bf91dc55bdf70