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An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Authors :
Wai Chung Fu
Chung-Hang Leung
Dik-Lung Ma
Daniel Shiu-Hin Chan
Ka-Ho Leung
Hong-Zhang He
Source :
Sensors and Actuators B: Chemical. 177:487-492
Publication Year :
2013
Publisher :
Elsevier BV, 2013.

Abstract

We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution.

Details

ISSN :
09254005
Volume :
177
Database :
OpenAIRE
Journal :
Sensors and Actuators B: Chemical
Accession number :
edsair.doi...........1082ddd4c5b4de044331e4d33cbe26f2
Full Text :
https://doi.org/10.1016/j.snb.2012.11.053