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Differentiation of Canine Bone Marrow Mesenchymal Stem Cells into Islet-Like Cells
- Source :
- Applied Biological Research. 20:118
- Publication Year :
- 2018
- Publisher :
- Diva Enterprises Private Limited, 2018.
-
Abstract
- The present study was aimed to study in vitro differentiation of canine bone marrow mesenchymal stem cells (BMSCs) to islet-like cells of pancreas. BMSCs were isolated from healthy dogs and cultured in laboratory. BMSCs after 3rd passage (at 80% confluence) were induced to differentiate into isletlike cells by using 2-step induction methods. Characterization of differentiated cells of BMSC into islet-like cells was done by observing cell morphology, expression of canine insulin gene by reverse transcriptase PCR (RT-PCR) and quantitative PCR (qPCR), detection of nestin and insulin proteins by immunocytochemistry and quantification of insulin level in culture media. Typical grape-like clusters of cells were observed under bright field microscope. Expression of insulin gene and protein was observed in differentiated cells. Insulin level in culture media of differentiated cells was significantly high. It was concluded that canine BMSCs could effectively be differentiated into islet-like cells. These differentiated cells could play an important role in diabetic therapy of dogs.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Insulin
medicine.medical_treatment
Cellular differentiation
Immunocytochemistry
Nestin
Biology
Cell morphology
Molecular biology
In vitro
Reverse transcription polymerase chain reaction
03 medical and health sciences
030104 developmental biology
medicine.anatomical_structure
medicine
Pediatrics, Perinatology, and Child Health
Pancreas
Subjects
Details
- ISSN :
- 09744517 and 09720979
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Applied Biological Research
- Accession number :
- edsair.doi...........10924361640ff135eb95e2b755b819b9