Simultaneous Determination of Senecionine, Senlciphylline, and Senecionine N-Oxide inGynura segetumby RP-HPLC

A rapid reversed-phase high-performance liquid chromatographic (RP-HPLC) method was established for simultaneous determination of senecionine, senlciphylline, and senecionine N-oxide in a famous traditional Chinese medicine, Gynura segetum, which has been commonly used for hemostasis. The HPLC assay was performed on a Kromasil KR100-5 C18 column (25 cm × 4.6 mm, 5 µm) with mobile phase composed of acetonitrile and 0.2% phosphoric acid–triethylamine within 40 min. The detection wavelength was 220 nm. All the compounds showed good linearity (r2 > 0.9997). The method was reproducible with intra- and interday variation less than 2.82%. The recovery of the assay was in the range of 96.55–103.88%. The method was successfully applied to the quantification of three constituents in 15 Gynura segetum samples collected from different metropolis. The results indicated that the developed assay could be considered as a suitable quality-control method for Gynura segetum.