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Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode

Authors :
Husam M. Abu-Soud
Roohi Jeelani
Sana N. Khan
Source :
Methods in Molecular Biology ISBN: 9781493976942
Publication Year :
2018
Publisher :
Springer New York, 2018.

Abstract

Precise information about the intracell nitric oxide (NO) concentration [NO] of a single cell are necessary in designing accurate experiments to further knowledge and develop treatment plans in certain disorders. The direct quantitative measurement of [NO] in situ in an intact cellular complex should be useful in tracking real-time and rapid changes at nanomolar levels. In this work, we describe the direct, real-time, and quantitative intracellular [NO] measurement utilizing an L-shaped amperometric integrated NO-selective electrode. This method not only provides an elegant and convenient approach to real-time the measurement of NO in physiological environments but also mimics the loss of NO caused by rapid NO diffusion combined with its reactivity in the biological milieu.

Details

ISBN :
978-1-4939-7694-2
ISBNs :
9781493976942
Database :
OpenAIRE
Journal :
Methods in Molecular Biology ISBN: 9781493976942
Accession number :
edsair.doi...........12c55a52f528f2fe53e4eeba61838a20
Full Text :
https://doi.org/10.1007/978-1-4939-7695-9_2