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Low-Frequency Electrical Stimulation Reduces the Impairment in Synaptic Plasticity Following Epileptiform Activity in Rat Hippocampal Slices through α1, But Not α2, Adrenergic Receptors
- Source :
- Neuroscience. 406:176-185
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Low frequency stimulation (LFS) has anticonvulsant effect and may restore the ability of long-term potentiation (LTP) to the epileptic brain. The mechanisms of LFS have not been completely determined. Here, we showed that LTP induction was impaired following in vitro epileptiform activity (EA) in hippocampal slices, but application of LFS prevented this impairment. Then, we investigated the involvement of α-adrenergic receptors in this effect of LFS. EA was induced by increasing the extracellular K+ concentration to 12 mM and EPSPs were recorded from CA1 neurons in whole cell configuration. EA increased EPSP amplitude from 6.9 ± 0.7 mV to 9.6 ± 0.6 mV. For LTP induction, the Schaffer collaterals were stimulated by high frequency stimulation (HFS; two trains of 100 pulses, 100 Hz at the interval of 20 s). The application of HFS resulted in 40.9 ± 2.3% increase in the amplitude of EPSPs. However, following EA, HFS could not produce any significant changes in EPSP amplitude. Administration of LFS (1 Hz, 900 pulses) to Schaffer collaterals at the beginning of EA restored LTP induction to the hippocampal slices and HFS increased the EPSPs amplitude up to 41.7 ± 3.1% of baseline. When slices were perfused by prazosin (α1-adrenergic receptor antagonist; 10 μM) before and during LFS application, LFS improvement on LTP induction was reduced significantly. Perfusion of slices by yohimbine (α2-adrenergic receptor antagonist; 5 μM) had no effect on LFS action. Therefore, it may be concluded that following epileptiform activity, LFS can improve the impairment of LTP generation through α1, but not α2, adrenergic receptor activity.
- Subjects :
- 0301 basic medicine
Adrenergic receptor activity
medicine.medical_specialty
Chemistry
musculoskeletal, neural, and ocular physiology
General Neuroscience
Long-term potentiation
Stimulation
Hippocampal formation
Yohimbine
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Endocrinology
nervous system
Internal medicine
Brain stimulation
Prazosin
medicine
LTP induction
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 03064522
- Volume :
- 406
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi...........12fcf45ff70516253a6d9e103ac26f43