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Purification of PP2Ac from Bovine Heart

Authors :
Hue T. Tran
Tony Ferrar
Greg B. G. Moorhead
Anne Ulke-Lemée
Publication Year :
2007
Publisher :
Humana Press, 2007.

Abstract

The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.

Subjects

Subjects :
Chromatography
Ion exchange
Chemistry
Protein subunit
Size-exclusion chromatography
Ethanol precipitation
Ammonium sulfate precipitation
Catalysis

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........1541ab14558e33f81484f68d30fe8431

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