The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D-Dimer

ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular-oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D-dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D-dimer concentrations with fast response time of ca. 20 s.