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Determination of Fusarium mycotoxins by liquid chromatography/tandem mass spectrometry coupled with immunoaffinity extraction
- Source :
- Rapid Communications in Mass Spectrometry. 24:2445-2452
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- A method for the simultaneous quantitative determination of deoxynivalenol (DON), T-2 toxin (T-2), HT-2 toxin (HT-2) and zearalenone (ZEN) in wheat and biscuit by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) coupled with immunoaffinity extraction is described. A clean-up was carried out using a DZT MS-PREP® immunoaffinity column (IAC), and the effect of the sample dilution rate and sample loading was investigated. Furthermore, the effects of ion suppression of a multifunctional column (MFC) and the IAC in the clean-up were compared. The results with the DZT MS-PREP® IAC showed that it is possible to make the sample dilution rate low, and indicated a higher solvent-tolerance than usual with an IAC. Sample loading was optimized at 0.25 g. Ion suppression was lowered by purification of the toxins using the DZT MS-PREP® IAC. Recoveries of each mycotoxin from wheat and biscuit samples spiked at two levels ranged from 78 to 109%. The limits of detection in wheat and biscuit was in the range of 0.03–0.33 ng·g−1. From these studies, it is suggested that use of an IAC is effective in the clean-up of each mycotoxin, and, when combined with LC/ESI-MS/MS, it is good for the determination of mycotoxins in foodstuffs due to its rapidity and high sensitivity. Copyright © 2010 John Wiley & Sons, Ltd.
- Subjects :
- Detection limit
Chromatography
Electrospray ionization
Organic Chemistry
Extraction (chemistry)
Ion suppression in liquid chromatography–mass spectrometry
Tandem mass spectrometry
Analytical Chemistry
chemistry.chemical_compound
chemistry
Liquid chromatography–mass spectrometry
Mycotoxin
Zearalenone
Spectroscopy
Subjects
Details
- ISSN :
- 09514198
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Rapid Communications in Mass Spectrometry
- Accession number :
- edsair.doi...........15b4a2ffad7751ecfcdc7b81bca13f65