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Mapping QTLs for Witches' Broom (Crinipellis Perniciosa) Resistance in Cacao (Theobroma Cacao L.)
- Source :
- Euphytica. 149:227-235
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Molecular markers (RAPD, AFLP and microsatellites) were used to generate a linkage map and to identify QTLs associated to witches' broom (Crinipellis perniciosa) resistance in cacao (Theobroma cacao), using 82 individuals of an F2 population derived from the clones ICS-1 (susceptible) and Scavina-6 (resistant). Fifteen evaluations of the number of brooms have been carried out in six years (1997–2002). In order to increase the precision and accuracy in the measures of resistance, each F2 plant was cloned in three replications in a randomized block design with single-tree plots and evaluated over 2 years. Three hundred and forty-two markers were obtained, being 33 microsatellites, 77 AFLPs and 232 RAPDs. The distribution of the number of brooms in the F2 population was skewed to resistance, suggesting the involvement of major genes controlling resistance and the repeatability estimated for resistance was 44%. A strong putative QTL was detected as being related to witches' broom resistance. Associated to this QTL, the microsatellite mTcCIR35 explained 35.5% of the phenotypic variation in resistance. This marker is being used for marker-assisted selection in Scavina-6 progenies, including those selected in private plantations, as an auxiliary tool to the phenotypic selection.
- Subjects :
- Genetics
biology
Theobroma
Broom
food and beverages
Plant Science
Horticulture
Plant disease resistance
Quantitative trait locus
biology.organism_classification
RAPD
Crinipellis
chemistry.chemical_compound
chemistry
Molecular marker
Amplified fragment length polymorphism
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 15735060 and 00142336
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- Euphytica
- Accession number :
- edsair.doi...........15cd918d6cf328dd569c43b2aaa4be54