Tumor B7-H1 regulates cancer stem cell generation and virulence

Authors :: Harshita B. Gupta
Vincent Hurez
Curtis A Clark
Gangadhara R Sareddy
Ratna Vadlamudi
Rong Li
Tyler J Curiel
Source :: The Journal of Immunology. 196:72.3-72.3
Publication Year :: 2016
Publisher :: The American Association of Immunologists, 2016.
Abstract: Cancer stem cells (CSC) resist chemotherapy and radiation, leading to treatment failures and reduced patient survival. Thus, CSC are clinically relevant therapy targets. B7-H1 is an immune checkpoint molecule and a target for αB7-H1 immunotherapy. We generated control (native B7-H1+) and B7-H1lo B16 melanoma lines by shRNA. B16*ctrl cultures were 4.2% CSCs (CD44+CD133+CD24+) vs. 0.19% in B16*B7-H1lo. CSC from B16*B7-H1lo cultures formed fewer and smaller (