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[Untitled]

Authors :
Kai Koo
P.M. Foegeding
Harold E. Swaisgood
Source :
Biotechnology Techniques. 12:549-552
Publication Year :
1998
Publisher :
Springer Science and Business Media LLC, 1998.

Abstract

A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RT-PCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing. © Rapid Science Ltd. 1998

Subjects

Subjects :
chemistry.chemical_compound
Subcloning
Biochemistry
chemistry
Nucleic acid
RNA
Biology
Isolation (microbiology)
Applied Microbiology and Biotechnology
Molecular biology
DNA
DNA sequencing

Details

ISSN :
0951208X
Volume :
12
Database :
OpenAIRE
Journal :
Biotechnology Techniques
Accession number :
edsair.doi...........1a23d34091796f8735fc2aca52a966b0
Full Text :
https://doi.org/10.1023/a:1008859632378
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