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Suitability of Tisochrysis lutea at different growth phases as an enrichment diet for Brachionus plicatilis sp. complex rotifers
- Source :
- Journal of Applied Phycology. 32:3933-3947
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- In larviculture of marine finfish, Tisochrysis lutea cultured cells have a potential to be a sustainable enrichment diet for rotifers due to its de novo synthesis of docosahexaenoic acid (DHA). To improve its effectiveness in rotifer enrichment for later larviculture, we determined which growth phase of T. lutea can enhance DHA in polar lipids (PLs) in Brachionus plicatilis sp. complex rotifers without reducing their vigor. Tisochrysis lutea was harvested at logarithmic growth phase, deceleration growth phase, and stationary phase. The ambient concentrations of phosphorus (P) and nitrogen (N), and fatty acid composition of microalgae were measured. P was limited between logarithmic and deceleration growth phases, followed by N starvation in stationary phase. Total lipid DHA was lowest in logarithmic growth phase, whereas DHA levels increased in deceleration growth phase and maintained at high levels even in stationary phase. PL-DHA also increased during deceleration growth phase, although its abundance dropped during stationary phase. In a rotifer enrichment trial, T. lutea harvested in each phase were fed to B. plicatilis sp. complex rotifers for 12 h. The highest PL-DHA value for cells in deceleration growth phase reflected the concentrations of the rotifers. No significant differences were found in rotifer growth rate and egg ratio. Rotifers fed cells in deceleration growth phase exhibited higher swimming speeds than those fed the other cells, potentially linking to increased larval capture success. Therefore, we recommend the deceleration growth phase as an optimal timing for T. lutea harvest for the effective enrichment of B. plicatilis sp. complex rotifers.
- Subjects :
- 0106 biological sciences
Larva
biology
010604 marine biology & hydrobiology
Phosphorus
chemistry.chemical_element
Plant physiology
Rotifer
Plant Science
Aquatic Science
Brachionus
biology.organism_classification
01 natural sciences
De novo synthesis
Animal science
chemistry
Docosahexaenoic acid
Growth rate
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15735176 and 09218971
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Phycology
- Accession number :
- edsair.doi...........1ba4805452fb83229df4ae5a5c6b1aa5