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Conversion Tract Analysis of Homology-Directed Genome Editing Using Oligonucleotide Donors
- Source :
- Methods in Molecular Biology ISBN: 9781493994991
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- Homology-directed genome editing is the intentional alteration of an endogenous genetic locus using information from an exogenous homology donor. A conversion tract is defined as the amount of genetic information that is converted from the homology donor to a given strand of the targeted chromosomal locus. Because of this, conversion tract analysis retrospectively not only elucidates the mechanism of homology-directed genome editing but also provides valuable insights on the conversion efficiency of every nucleotide in the homology donor. Here we describe a blue fluorescent protein-to-green fluorescent protein conversion system that can be conveniently used to measure the efficiency and analyze the lengths of conversion tracts of homology-directed genome editing using oligonucleotide donors in mammalian cells.
- Subjects :
- chemistry.chemical_classification
0303 health sciences
Oligonucleotide
Locus (genetics)
Computational biology
Biology
Homology (biology)
03 medical and health sciences
0302 clinical medicine
Genome editing
chemistry
Fluorescent protein
Nucleotide
Gene conversion
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-4939-9499-1
- ISBNs :
- 9781493994991
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493994991
- Accession number :
- edsair.doi...........1c26a601acaba2feed74c11fb6b8842f