Back to Search Start Over

Molecular basis of RhD-positive/D-negative chimerism in two patients

Authors :
Eid Ss
Source :
Eastern Mediterranean Health Journal. 10:228-241
Publication Year :
2004
Publisher :
World Health Organization Regional Office for the Eastern Mediterranean (WHO/EMRO), 2004.

Abstract

This study investigated two patients with Rh chimerism:patient A, a healthy individual, and patient B with myelofibrosis. Flow cytometry studies showed two red blood cell populations of Rh phenotypes R1r and rr at percentages of about 25% and 75% respectively. Normal RhD transcript sequences were found following RT-PCR. Genomic DNA [gDNA] showed normal exon, intron, GATA regions and exon/intron boundary sequences except for a single base change in intron 7 [C –>A] of exon 7 in patient A. The major change found in both patients was the absence of RHD exon 9 DNA in gDNA isolated from peripheral blood. These findings suggest a somatic mutation, probably in a stem cell common to the myeloid lineage of both patients, and indicate that patient A may undergo malignant transformation in the future

Details

ISSN :
16871634 and 10203397
Volume :
10
Database :
OpenAIRE
Journal :
Eastern Mediterranean Health Journal
Accession number :
edsair.doi...........1c41e606a1ca891a81d7195f47a4dd79
Full Text :
https://doi.org/10.26719/2004.10.1-2.228