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Prototype of a DNA Vaccine against Marburg Virus
- Source :
- Bulletin of Experimental Biology and Medicine. 170:475-478
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- The preparation and study of the biological properties of the pVAKS-GPVM DNA immunogen containing a gene encoding Marburgvirus glycoprotein are described. The specificity of blood serum antibodies of guinea pigs immunized with DNA immunogen was analyzed by ELISA. Inactivated viral preparation, recombinant glycoprotein (GP) obtained in the prokaryotic system and virus-like particles based on the recombinant vesicular stomatitis virus exhibiting Marburgvirus GP were used as the antigens. The neutralizing activity of antibodies of immunized animals was tested in vitro using a pseudovirus system. It was demonstrated that the developed immunogen administered to guinea pigs induced the production of specific antibodies that neutralize virus-like particles and Marburgvirus in cultured Vero cells.
- Subjects :
- 0301 basic medicine
Immunogen
biology
General Medicine
Marburgvirus
biology.organism_classification
Virology
General Biochemistry, Genetics and Molecular Biology
DNA vaccination
law.invention
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Blood serum
Antigen
Vesicular stomatitis virus
law
Vero cell
Recombinant DNA
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15738221 and 00074888
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Bulletin of Experimental Biology and Medicine
- Accession number :
- edsair.doi...........1dc4ff69792b2f070be51de0dda50f97