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Chemische Charakterisierung desEscherichia coli K 1 Kapselpolysaccharides

Authors :
Siegbert Jahn
Ruediger Von Prof Baehr
Roland Grunow
Roland Starke
Bettina Kissig
G. Schmidt
M. Mehl
Source :
Journal of Basic Microbiology. 29:221-232
Publication Year :
1989
Publisher :
Wiley, 1989.

Abstract

The exact chemical characterization of the prepared Escherichia coli K 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti-K 1-antibodies. The K 1-antigen, prepared by phenol-water-extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared- and 13C-NMR-spectroscopy, and gelchromatography. After testing the serological specificity, the K 1-antigen was identified as a high molecular polyneuraminic acid.

Details

ISSN :
15214028 and 0233111X
Volume :
29
Database :
OpenAIRE
Journal :
Journal of Basic Microbiology
Accession number :
edsair.doi...........1f4918eaeee6cd9072eb7e06444e57c9
Full Text :
https://doi.org/10.1002/jobm.3620290408