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Sphingolipid Control of Fibroblast Heterogeneity Revealed by Single-Cell Lipidomics

Authors :
Gian Paolo Dotto
Laura Capolupo
Shane R. Ellis
Romain Guiet
Jonathan Paz Montoya
Giovanni D'Angelo
Irina Khven
Francesco Russo
Sylvia Ho
Andrew P. Bowman
Dhaka Ram Bhandari
Johannes Müthing
Gioele La Manno
Galina Glousker
Bernhard Spengler
Ron M. A. Heeren
Luigi Mazzeo
Publication Year :
2021
Publisher :
Cold Spring Harbor Laboratory, 2021.

Abstract

Human cells produce thousands of lipids that impact a wide range of biological processes in ways we are only starting to characterize. The cellular composition in lipids changes during differentiation events and also varies across individual cells of the same type. Yet, the precise differences in lipid composition that directly affect cell phenotypes remain unknown. Here we have measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging to single-cell transcriptomics. We found that the cell-to-cell variation of specific lipid metabolic pathways contributes to the establishment of cell states involved in wound repair and in skin cancer growth. Sphingolipid composition defined fibroblast subpopulations while sphingolipid metabolic rewiring drove cell state transitions. These data uncover a role for cell-to-cell lipid heterogeneity in the determination of cell states and reveal a new regulatory component to the homeostasis and self-organization of multicellular systems.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........1f79a1935dd44370b594ae3719be7472