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Additional file 5: Figure S5. of PKM2 promotes tumor angiogenesis by regulating HIF-1α through NF-κB activation
- Publication Year :
- 2016
- Publisher :
- Figshare, 2016.
-
Abstract
- PKM2 regulates hypoxia-induced VEGF promoter activity. A, PaTu2 cancer cells were transiently transfected with 3xHRE-luc and pTK-Renilla. Four hours after transfection cells were incubated under normoxic or hypoxic conditions in the absence or presence of 30 μM TEPP-46. Cell lysates were subjected to luciferase assay. Bars are the means +/- SEM of at least two independent experiments performed in duplicate. B, cancer cells were transiently transfected with VEGF-luc and pTK-Renilla. Four hours after transfection cells were incubated under normoxic or hypoxic conditions in the absence or presence of 30 μM TEPP-46. Cell lysates were subjected to luciferase assay. Bars are the means +/- SEM of at least two independent experiments performed in duplicate. C, D, PaTu2 and Capan1 cells were transiently transfected with VEGF-luc reporter. Four hours later cells were incubated under normoxic or hypoxic conditions in the absence or presence of 10 μM BAY 87-2243. Lysates were subjected to luciferase assay. Bars are the means +/- SEM of at least two independent experiments performed in duplicate (No – normoxia; Hy – hypoxia). (PPTX 138 kb)
- Subjects :
- animal structures
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........204b6950d6f694ac8cd4d864c5307507
- Full Text :
- https://doi.org/10.6084/m9.figshare.c.3698878_d2.v1