Single Molecule Force Spectroscopy on Polyelectrolytes: Effect of Spacer on Adhesion Force and Linear Charge Density on Rigidity

We have synthesized a new kind of poly(2-acrylamido-2-methylpropanesulfonic acid) with crown ether and studied comparatively the single molecule force spectroscopy of the polymers with and without crown ether, in terms of desorption and elongation. The smooth desorption process enabled us to calculate the loading rate of the stretching process. For the two polymers, desorption forces were loading rate independent and ionic strength insensitive. Interestingly, the desorption forces of the two polymers were undistinguishable in all conditions. These findings demonstrate (1) the polymer chains adopt a trainlike (flat) conformation at the interface with a high adsorption/desorption rate, (2) the spacer, which separates the charged group from the hydrophobic backbone and combines the two properties together, should account for the retained desorption force at high salt concentration, and (3) the 20% less in linear charge density does not affect the desorption force remarkably since hydrophobic interaction domi...