A mini-primer set in a multiplex PCR fashion covering the MTDNA control region from submerged skeletal remains

When DNA is recovered in a small quantity and low quality, typing methods based on mtDNA should be considered. Mini-primer sets multiplex with reduced amplicons has been successful in amplifying the entire CR. This study developed a mini-primer set PCR multiplex assay to amplify the entire CR of mtDNA from fragments of human skeletal remains submerged in water up to 30, 60 and 90days. So far, the new mini-primer set for PCR multiplex assay was tested on four samples, three of them submerged in river water for a period of 30 months. This approach gave successful PCR amplifications for the three samples submerged and the control. The following sequencing analysis brought partial mtDNA control region profiles to the samples and a complete to the control.