Back to Search Start Over

Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase

Authors :
Sneha Parmar
Samuel J. Gonzalez
Julia M. Heckel
Soumya Mukherjee
Mark McClellan
Duncan J. Clarke
Marnie Johansson
Damien Tank
Athena Geisness
David K. Wood
Melissa K. Gardner
Source :
Journal of Cell Biology. 222
Publication Year :
2023
Publisher :
Rockefeller University Press, 2023.

Abstract

During mitosis, sister chromatids are stretched apart at their centromeres via their attachment to oppositely oriented kinetochore microtubules. This stretching generates inwardly directed tension across the separated sister centromeres. The cell leverages this tension signal to detect and then correct potential errors in chromosome segregation, via a mechanical tension signaling pathway that detaches improperly attached kinetochores from their microtubules. However, the sequence of events leading up to these detachment events remains unknown. In this study, we used microfluidics to sustain and observe low-tension budding yeast metaphase spindles over multiple hours, allowing us to elucidate the tension history prior to a detachment event. We found that, under conditions in which kinetochore phosphorylation weakens low-tension kinetochore-microtubule connections, the mechanical forces produced via the dynamic growth and shortening of microtubules is required to efficiently facilitate detachment events. Our findings underscore the critical role of robust kinetochore microtubule dynamics in ensuring the fidelity of chromosome segregation during mitosis.

Subjects

Subjects :
Cell Biology

Details

ISSN :
15408140 and 00219525
Volume :
222
Database :
OpenAIRE
Journal :
Journal of Cell Biology
Accession number :
edsair.doi...........22dd67b4f2f5ae879da2af9df644cc73
Full Text :
https://doi.org/10.1083/jcb.202202085