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Emission Detection of Mercuric Ions in Aqueous Media Based-on Dehybridization of DNA Duplexes

Authors :
Ka Young Kim
Byul Nim Oh
Jin-Ah Kim
Hee Kyung Kang
Jinheung Kim
Qiong Wu
Eswaran Rajkumar
Mi Sun Cha
Source :
Bulletin of the Korean Chemical Society. 32:3223-3228
Publication Year :
2011
Publisher :
Korean Chemical Society, 2011.

Abstract

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as , , , , , , , , , , , , had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.

Details

ISSN :
02532964
Volume :
32
Database :
OpenAIRE
Journal :
Bulletin of the Korean Chemical Society
Accession number :
edsair.doi...........234b845071e17a4c2d28ffd13cd8fcd7
Full Text :
https://doi.org/10.5012/bkcs.2011.32.9.3223