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Effect of a Stabilized Microbubble Contrast Agent on CW Ultrasound Induced Red Blood Cell Lysis In Vitro
- Source :
- Echocardiography. 12:1-11
- Publication Year :
- 1995
- Publisher :
- Wiley, 1995.
-
Abstract
- Human red blood cells (RBCs) in vitro at various fractional hematocrits (HCTs) were exposed for 60–120 seconds in a dialysis tubing vessel to 1 MHz continuous wave ultrasound (0–5 W/cm2 SPTA intensity); exposure vessels were either rotated at 200 rpm or stationary. Some RBC suspensions also contained Albunex® (ALX; a commercially-produced microbubble clinical ultrasound contrast agent) at final concentrations ranging from 0–41 μL/mL RBC suspension. Isonation was either by one transducer or by two opposing, continuously-gated, balanced transducers. For the vessel rotation / no rotation experiments, ultrasound-induced cell lysis was always increased with the ALX regimen relative to that of the no-ALX regimen at HCTs up to about 10%; at higher HCTs (including whole blood), ultrasound-induced hemol-ysis essectially ceased to occur. The data are consistent with reports of the ineffectiveness of continuous wave ultrasound at 3–5 W/cm2 to lyse cells in vitro at physiological cell densities with or without echocontrast medium supplemantation.
- Subjects :
- Pathology
medicine.medical_specialty
Lysis
business.industry
Ultrasound
In vitro
Dialysis tubing
Intensity (physics)
Red blood cell
Clinical ultrasound
medicine.anatomical_structure
medicine
Radiology, Nuclear Medicine and imaging
Cardiology and Cardiovascular Medicine
business
Biomedical engineering
Whole blood
Subjects
Details
- ISSN :
- 15408175 and 07422822
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Echocardiography
- Accession number :
- edsair.doi...........236cab4c4fc6d3de9cdcfd4f36b412e1