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Determination ofStaphylococcus aureusB-1266 by an Enzyme-Free Electrochemical Immunosensor Incorporating Magnetite Nanoparticles
- Source :
- Analytical Letters. 50:924-935
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- A simple and inexpensive immunosensor is reported for the rapid determination of Staphylococcus aureus B-1266 that uses Fe3O4–SiO2–NH2 nanoparticles as the direct signal label. The electrochemical immunoassay procedure includes the incubation of bacteria with excess magnetite nanoparticles, the magnetic separation of the free nanoparticles, a labeled immunocomplex formation on the surface of a planar electrode, and the electrochemical response from the magnetite nanoparticles in the immunocomplex. The electrochemical immunosensor allows for the selective and accurate detection of S. aureus from 10 to 105 CFU mL−1 with a relative standard deviation lower than 10%. The limit of detection was 8.7 CFU mL−1.
- Subjects :
- Detection limit
Chromatography
Chemistry
010401 analytical chemistry
Biochemistry (medical)
Clinical Biochemistry
Magnetic separation
Nanoparticle
Enzyme free
02 engineering and technology
021001 nanoscience & nanotechnology
medicine.disease_cause
Electrochemistry
01 natural sciences
Biochemistry
0104 chemical sciences
Analytical Chemistry
Magnetite Nanoparticles
Staphylococcus aureus
Electrochemical immunoassay
medicine
0210 nano-technology
Spectroscopy
Subjects
Details
- ISSN :
- 1532236X and 00032719
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Analytical Letters
- Accession number :
- edsair.doi...........237ed035661debc16dd8dffdf2983ba1