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Isolation of Acidic Mucilage from the Outer Seed Coat of Shaddock (Citrus grandis Osbeck) and Evaluation of Its Functional Properties
- Source :
- American Journal of Plant Sciences. :780-788
- Publication Year :
- 2016
- Publisher :
- Scientific Research Publishing, Inc., 2016.
-
Abstract
- After water imbibition, the outer layer seed coat of shaddock (Citrus grandis Osbeck) produces transparent gel-like mucilage (MSS), but its characteristics have never been studied before. This study aimed to assess the physico-chemical and functional properties of MSS. Extractions of MSS with deionized water at room temperature yielded about 3.5% based on the dry weight of seed. The major components were neutral sugars and uronic acids in the amounts of 33.5% and 49.6%, respectively. The acidic nature of MSS was confirmed by ruthenium red staining. Its water holding capacity and viscosity were 44.53 g•g-1 DW and 1660 cP at 10 g/L, respectively. MSS showed a weak quenching activity against DPPH radical, and moderate ferrous ion-chelating and superoxide anion radical scavenging activities, with IC50 value of 1.5 g/L and 1.1 g/L, respectively. A methyl thiazolyl tetrazolium (MTT) assay demonstrated that MSS significantly stimulated the viability of mouse skin fibroblasts (NIH/3T3) at 5 - 300 mg/L. These results impart the potential usefulness of the MSS to food, cosmetics and other applications.
- Subjects :
- 0106 biological sciences
Ruthenium red
Quenching (fluorescence)
DPPH
04 agricultural and veterinary sciences
General Medicine
040401 food science
01 natural sciences
digestive system diseases
Staining
Ferrous
chemistry.chemical_compound
0404 agricultural biotechnology
chemistry
Mucilage
Dry weight
Biochemistry
Imbibition
010606 plant biology & botany
Nuclear chemistry
Subjects
Details
- ISSN :
- 21582750 and 21582742
- Database :
- OpenAIRE
- Journal :
- American Journal of Plant Sciences
- Accession number :
- edsair.doi...........25d9ac665b11eddf569d015b28aa46a8