Back to Search
Start Over
Detection of Antimicrobial Resistance-Related Changes in Biochemical Composition of Staphylococcus aureus by Means of Atomic Force Microscopy-Infrared Spectroscopy
- Source :
- Analytical Chemistry. 91:15397-15403
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- The development of antimicrobial resistance (AMR) resulting from widespread antibiotic usage is occurring at an alarming pace, much faster than our understanding of the mechanisms behind resistance. Knowledge about resistance-related phenotypic and genotypic changes is critical for the development of new drugs. Here, we identify changes in the chemical composition of Staphylococcus aureus associated with the development of resistance to last resort drugs, vancomycin and daptomycin, using a novel, single cell, nanoscale technique, atomic force microscopy-infrared spectroscopy (AFM-IR), combined with chemometric analysis. We utilized paired clinical isolates, with the parent (susceptible) strain isolated prior to treatment and the daughter (resistant) strain obtained from the same patient after drug admission and clinical failure. We observed an increase in the amount of nonintracellular carbohydrates, indicating thickening or changes in the packing of the cell wall, as well as changes in the phospholipid content in relation to vancomycin resistance and daptomycin nonsusceptibility, respectively.
- Subjects :
- Drug
Strain (chemistry)
medicine.drug_class
Chemistry
media_common.quotation_subject
010401 analytical chemistry
Antibiotics
010402 general chemistry
medicine.disease_cause
01 natural sciences
0104 chemical sciences
Analytical Chemistry
Microbiology
Cell wall
Antibiotic resistance
Staphylococcus aureus
medicine
Vancomycin
Daptomycin
media_common
medicine.drug
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi...........260046208d84a8d44a3f30a548d8492e