Flexural Strength and Cytotoxicity of ZCC, a Resin Modified Zinc-Calcium Liner Prototype

Objective: To investigate the flexural strength and cytotoxicity of ZCC, a resin modified zinc oxide-calcium carbonate liner prototype, compared with commercial dental liners. Materials and Methods: ZCC, Dycal, Ultra-Blend® plus (UB), and TheraCal LC® (TC) were evaluated for flexural strength. Six samples of each material were incubated in growth medium (10% fetal bovine serum supplemented DMEM) for 24 h. Primary human dental pulp cells were cultured in the conditioned medium from each sample, with growth media used as a control. Cytotoxicity was determined using an MTT assay. Data were analyzed by one-way ANOVA. The value of pppp>0.05). Conclusion: ZCC met the requirements for flexural strength per ISO 9917-2:2010(E). There was no significant difference in viability between the control and ZCC group at 24 and 48 hours.