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Clonal Propagation of Strawberry (Fragaria x ananassa Duch.) through In vitro Runner Tip Culture through Incorporation of Growth Hormones

Authors :
Shaikh Mizanur Rahman
Md. Nasir Uddin Badal
Md. Shoyeb
Md. Abdur Rauf Sarkar
Md. Shahedur Rahman
Source :
Biotechnology Journal International. :1-9
Publication Year :
2019
Publisher :
Sciencedomain International, 2019.

Abstract

Runner tips explants of strawberry give rise to multiple shoots when cultured on MS medium supplemented with different concentrations and combinations of BAP with KIN or NAA or GA3.The highest response of shoot multiplication was obtained on MS containing 2.5 mgl-1 BAP + 0.5 mgl-1 Kin + 0.5 mgl-1 GA3. The maximum frequency of rooting (83%) and highest number of roots (3.49) was produced in medium containing 1.0 mgl-1 IBA. The well grown rooted plantlets were acclimatized and successfully established in autoclaved vermiculate soil and as well as natural condition. Using our established protocol, it is also possible to provide large numbers of micropropagated plantlets of this cultivars to produce high quality strawberry fruit for commercial cultivation practices.

Details

ISSN :
24567051
Database :
OpenAIRE
Journal :
Biotechnology Journal International
Accession number :
edsair.doi...........26a41bb38bcf82a0964de0a4d17680b4
Full Text :
https://doi.org/10.9734/bji/2018/v22i430063