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Production and Characterization of Biosurfactant by Nocardia Species Isolated Form Soil Samples in Tehran

Production and Characterization of Biosurfactant by Nocardia Species Isolated Form Soil Samples in Tehran

Authors :
Razieh Mohammadzadeh
Mohamad Reza Pourmand
Abbas Morovvati
Ali Javadi
Zohre Baseri
Javad Hamedi
Fatemeh Gharebaghi
Seyyed Saeed Eshraghi
Hossein Ali Rahdar
Source :
Tenside Surfactants Detergents. 58:74-80
Publication Year :
2021
Publisher :
Walter de Gruyter GmbH, 2021.

Abstract

In this work, we report the Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The biosurfactant production from the Nocardia species was determined by qualitative standard methods. The selected bacterial strain had better oil spreading and emulsifying activity and surface tension reduction. The biosurfactant producer strain was identified by 16S rRNA gene sequencing method. Using Fourier transform infrared spectroscopy and comparative analysis, the biosurfactant structure could be detected. Oil spreading tests and blue agar plate tests confirmed the presence of biosurfactant and extracellular anionic glycolipids. The E24% assay using olive oil showed strong emulsifying characteristic of extracted biosurfactant with a 100%. The stability of the biosurfactant produced in different conditions was significant. Nocardia coubleae was identified by biochemical methods and molecular methods.

Details

ISSN :
21958564 and 09323414
Volume :
58
Database :
OpenAIRE
Journal :
Tenside Surfactants Detergents
Accession number :
edsair.doi...........272ca3271c21a47a2ccb4276f7892bf8
Full Text :
https://doi.org/10.1515/tsd-2020-2263