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Activated naïve B cells promote development of malignant pleural effusion by differential regulation of TH1 and TH17 response
- Source :
- American Journal of Physiology-Lung Cellular and Molecular Physiology. 315:L443-L455
- Publication Year :
- 2018
- Publisher :
- American Physiological Society, 2018.
-
Abstract
- Inflammatory signaling networks between tumor cells and immune cells contribute to the development of malignant pleural effusion (MPE). B cells have been found in MPE; however, little is known about their roles there. In the present study, by using mouse MPE models, we noted that although the total B cells in MPE were decreased as compared with the corresponding blood and spleen, the percentage of activated naïve B cells expressing higher levels of CD80, CD86, myosin heavy chain-II, CD44, CD69, and programmed cell death-ligand 1 (PD-L1) molecules were increased in wild-type mouse MPE. Compared with wild-type mice, decreased T helper (TH)1 cells and increased TH17 cells were present in B cell-deficient mouse MPE, which paralleled to the reduced MPE volume and longer survival time. Adoptive transfer of activated naïve B cells into B cell-deficient mice was able to increase TH1 cells and decrease TH17 cells in MPE and shorten the survival of mice bearing MPE. Furthermore, we demonstrated that activated naïve B cells inhibited TH17-cell expansion via the PD-1/PD-L1 pathway and promoted naïve CD4+ T-cell differentiation into TH1/TH17 cells through secreting IL-27/IL-6 independent of the PD-1/PD-L1 pathway. Collectively, our data uncovered a mechanism by which naïve B cells promote MPE formation by regulating TH1/TH17 cell responses, making these B cells an attractive target for therapeutic intervention in the fight against cancer.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
CD86
Adoptive cell transfer
biology
Physiology
Chemistry
Cell
Naive B cell
CD44
Spleen
Cell Biology
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Immune system
Physiology (medical)
Cancer research
medicine
biology.protein
CD80
030215 immunology
Subjects
Details
- ISSN :
- 15221504 and 10400605
- Volume :
- 315
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Accession number :
- edsair.doi...........29449ea0337e22417eca68436baebeb9