Synthesis of Silver-Alginate Nanocomposites Colloidal Assisted by Microwave Irradiation as Antibacterial Material

Synthesis of silver-alginate nanocomposite colloidal as an antibacterial material has been done. The colloidal is prepared through a chemical reduction method assisted by microwave irradiation with alginat as a reducing agent and stabilizer. Silver nitrate is used as a precursor and NaOH as an accelerator. The formation of silver-nanoparticles was indicated using a UV-Vis spectrophotometer based on localized surface plasmon resonance (LSPR) phenomenon. The shape and size of silver nanoparticles were characterized using TEM. Effect of storage in ambient temperature of silver-alginate nanocomposites colloidal was carried out during 14 weeks. Investigation of antibacterial activity of silver-alginate nanocomposite was carried out using diffusion method. The results showed that, the concentration of NaOH, irradiation time, AgNO3 concentration affect to the absorption band of the LSPR which related with the number of nanoparticles produced. Based on the TEM images, the size of silver nanoparticles increased with increasing concentration of AgNO3. Silver-alginate nanocomposites was stable for 12 weeks based on the absorption band of the LSPR. The greater the concentration of silver nanoparticles in silver-alginate nanocomposites, tends to the higher their antibacterial properties.