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CCDC3 is specifically upregulated in omental adipose tissue in subjects with abdominal obesity

Authors :
Satoshi Ugi
Masatomo Rokushima
Osamu Sekine
Shiro Maeda
Hiroshi Maegawa
Atsunori Kashiwagi
Katsutaro Morino
Toru Tani
Hiroshi Yamamoto
Takeshi Yoshizaki
Yoshihiro Kawamura
Masaaki Kobayashi
Minako Imamura
Source :
Obesity. 22:1070-1077
Publication Year :
2013
Publisher :
Wiley, 2013.

Abstract

Objective The aim of this study was to search for novel markers of visceral adiposity. Methods Visceral (omental) and subcutaneous adipose tissues were obtained from 43 Japanese men. Microarray analysis using total RNA from visceral and subcutaneous adipose tissues obtained from five men with abdominal obesity and five nonobese men was first conducted. Then the expression pattern of candidate genes identified in the human study in mouse models of adiposity was examined. Results Among 30,500 genes evaluated, the mRNA expression of CCDC3 (encoding coiled-coil domain-containing protein 3) was upregulated in omental adipose tissues from abdominally obese subjects (3.07-fold) but not in subcutaneous adipose tissues (0.89-fold). Similar expression patterns were found in two distinct mouse models of obesity. In the analysis of all 43 men, CCDC3 mRNA levels in omental, but not in subcutaneous adipose tissue, were positively correlated with waist circumference and body mass index. CCDC3 was predicted to be a secretory protein, which was confirmed by western blotting, as overexpressed CCDC3 was secreted into the culture media. Conclusions The expression of CCDC3 is specifically increased in visceral adipose tissues in abdominally obese subjects. These results suggest that CCDC3 is a potential biomarker for estimating visceral adiposity.

Details

ISSN :
19307381
Volume :
22
Database :
OpenAIRE
Journal :
Obesity
Accession number :
edsair.doi...........2a9ee5157e7f560232f31463e407e596
Full Text :
https://doi.org/10.1002/oby.20645