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Synthesis of triacylglycerol containing conjugated linoleic acid by esterification using two blended lipases
- Source :
- Journal of the American Oil Chemists' Society. 83:35-38
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- We have developed an efficient esterification for the synthesis of triacylglycerol (TAG) containing conjugated linoleic acids (CLA) using a blend of two powdered lipases. Two pairs of blended lipases promoted the esterification. Rhizomucor miehei lipase, plus Alcaligenes sp. lipase and Penicillium cammembertii MAG and DAG lipase plus Alcaligenes sp. lipase were used. At the optmal ratio of two lipases, the content of TAG containing CLA (TAG-CLA) in all glycerols reached 82–83% after 47 h using 1 wt% of lipases. With R. miehei lipase plus Alcaligenes sp. lipase, the reaction time to obtain ca. 60% of TAG-CLA was one-third of that needed with R. miehei lipase alone. The optimal ratio of two lipases differed between these two pairs. The optimal ratio was 70–80 wt% of R. miehei lipase in the last stage of the reaction, whereas it was over a wide range of 10–90 wt% for P. camembertii lipase. In the blend of R. miehei lipase plus Alcaligenes sp. lipase, activity remained very high after 10 cycles of esterification (every 47 h) and could be used in the industrial production of TAG-CLA.
- Subjects :
- chemistry.chemical_classification
Diacylglycerol lipase
Chromatography
biology
General Chemical Engineering
Linoleic acid
Conjugated linoleic acid
Organic Chemistry
Triacylglycerol lipase
food and beverages
Rhizomucor miehei
biology.organism_classification
chemistry.chemical_compound
chemistry
Penicillium
biology.protein
Organic chemistry
lipids (amino acids, peptides, and proteins)
Lipase
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 0003021X
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Journal of the American Oil Chemists' Society
- Accession number :
- edsair.doi...........2b04e5cfe692dc4a510fa1473c487950