Phytosanitary status of grapevine in Montenegro

During 2006 and 2007, a survey on the incidence and distribution of fourteen grapevine viruses was carried out in the Skadar Lake basin, one of the two main grapevine-growing areas of Montenegro. In total 165 samples were collected from four red (‘Vranac’, ‘Kratosija’, ‘Merlot’ and ‘Cardinal’), two white (‘Chardonnay’ and ‘Rkaciteli’) and a few unknown grapevine varieties in the vicinity of Podgorica and Bar. The phytosanitary status of the collected samples was analysed by DAS-ELISA and the presence of Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine leafroll-associated virus 3 (GLRaV-3) was confirmed in some of them. The most frequently found virus in assayed samples was GLRaV-3 (54.5%), followed by GFLV (23%), GLRaV-1 (20%) and GLRaV-2 (0.6%). These serological analyses showed the absence of Grapevine leafroll-associated virus 6 (GLRaV-6), Grapevine leafroll-associated virus 7 (GLRaV-7), Raspberry bushy dwarf virus (RBDV), Strawberry latent ringspot virus (SLRSV), Tomato ringspot virus (ToRSV), Raspberry ringspot virus (RpRSV), Arabis mosaic virus (ArMV), Tobacco ringspot virus (TRSV), Tomato black ring virus (TBRV) and Cherry leaf roll virus (CLRV) from all tested samples.