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Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a22 176 and rhuZP3b177 348 and Their Mechanism
- Source :
- Journal of Andrology. 28:381-388
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- To explore the biological characteristics of the recombinant zona pellucida 3 (ZP3) peptides of rhuZP3a 22,176 and rhuZP3b 177,348 , we examined whether rhuZP3a 22,176 or rhuZP3b 177,348 trigger the acrosome reaction (AR) of human spermatozoa and we investigated the underlying mechanism. The assessment of AR was performed using chlortetracycline staining. The intracellular free calcium concentration ((Ca 2+ )i) in Fura-2/AM- loaded human sperm was monitored with a spectrofluorophotometer. We found that rhuZP3a 22,176 and rhuZP3b 177,348 were capable of eliciting AR at different concentrations. With the addition of either peptide, the (Ca 2+ )i level was raised to a peak with or without a plateau. The AR could be inhibited by pertussis toxin (PTX), EGTA, and pimozide (a T-type calcium channel blocker), whereas verapamil was less effective in this regard. The results of the present study suggest that peptides rhuZP3a 22,176 and rhuZP3b 177,348 have a role similar to human ZP3, and that the mechanism of the response to the peptides involves influx of calcium, the G protein pathway, and a T- type calcium channel.
- Subjects :
- medicine.medical_specialty
medicine.drug_class
Urology
Endocrinology, Diabetes and Metabolism
Calcium channel
Acrosome reaction
chemistry.chemical_element
Calcium channel blocker
Calcium
Biology
Pertussis toxin
Calcium in biology
Cell biology
EGTA
chemistry.chemical_compound
Endocrinology
medicine.anatomical_structure
Reproductive Medicine
chemistry
Internal medicine
medicine
Zona pellucida
Subjects
Details
- ISSN :
- 01963635
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Andrology
- Accession number :
- edsair.doi...........2be3c4a24632ae25308e959657807d24