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Culture Conditions for the Production of Alkaline Protease fromn-Paraffins by a Kabicidin Resistant Mutant No. 5–128B
- Source :
- Agricultural and Biological Chemistry. 38:657-661
- Publication Year :
- 1974
- Publisher :
- Oxford University Press (OUP), 1974.
-
Abstract
- A novel process for the microbial production of alkaline protease on an industrial scale was successfully established by using a kabicidin resistant mutant, No. 5–128B, derived from Fusarium sp. S–19–5. The most suitable carbon source for producing alkaline protease was n-paraffins (C10~C14) and the effective nitrogen source was dried-yeast cells containing no nucleic acid, the optimum concentrations being 12.5% (w/v) and 7.0% (w/v), respectively. The optimal temperature and initial pH for protease production were 24°C and 6.0, respectively. Under the optimal conditions using a shaker flask mutant No. 5–128B produced 41000 PU/ml of alkaline protease, which corresponded to about 10 times the amount produced by the parent strain. The relation between the high ability to produce alkaline protease and the resistance to kabicidin, a polyene antibiotic, is discussed.
- Subjects :
- Fusarium
Protease
Chromatography
biology
Strain (chemistry)
Chemistry
medicine.medical_treatment
Mutant
Alkaline protease
biology.organism_classification
Polyene
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Biochemistry
medicine
Nucleic acid
General Agricultural and Biological Sciences
Nitrogen source
Subjects
Details
- ISSN :
- 00021369
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Agricultural and Biological Chemistry
- Accession number :
- edsair.doi...........2cffc9040a37e05d4350bc89b200decf