FRET-Biosensoren: molekulare Werkzeuge in der Signalwegforschung

Genetically encoded, FRET based biosensors have revolutionized cell signalling research in the past decade. They allow tracking dynamic activities of signalling molecules such as protein kinases, Rho GTPases or second messengers in real time on a subcellular level. This article gives an overview about design, components and employment of different types of FRET biosensors. Exemplarily, a kinase activity reporter to visualize protein kinase D activity at the Golgi complex is presented.