Back to Search
Start Over
Expanding pH screening space using multiple droplets with secondary buffers for protein crystallization
- Source :
- Journal of Crystal Growth. 463:72-78
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- We have proposed a rational strategy for selecting a suitable pH of protein solution based on protein biochemical properties. However, it is difficult to use this strategy for biochemical properties unknown proteins. In this paper, a simpler and faster pH buffer strategy was proposed. An additional pH-controlling buffer was added to crystallization droplet mixed with protein solution and commercial crystallization reagents to adjust its pH. The results revealed that protein crystallization success rates were enhanced by this strategy due to expansion of the pH screening space, which was closely related with protein solubility. Thus, the possibility of reaching supersaturation was increased by using this strategy.
- Subjects :
- 0301 basic medicine
Supersaturation
Chromatography
Chemistry
010402 general chemistry
Condensed Matter Physics
Space (mathematics)
01 natural sciences
Protein solution
0104 chemical sciences
law.invention
Inorganic Chemistry
03 medical and health sciences
030104 developmental biology
Chemical engineering
law
Reagent
Materials Chemistry
Crystallization
Protein crystallization
Protein solubility
Subjects
Details
- ISSN :
- 00220248
- Volume :
- 463
- Database :
- OpenAIRE
- Journal :
- Journal of Crystal Growth
- Accession number :
- edsair.doi...........3166e9fcc2e84747112301c858a208ba