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A thiosemicarbazone derivative of the Vitamin K3 has been synthesized. Five transition metal complexes of the thiosemicarbazone have been prepared and characterized by i.r., u.v.–vis., molar conductance and thermal analyses. All of the complexes possess strong inhibitory action against G(+) Staphylococcus aureus, G(−) Hay bacillus, and G(−) Eschericha coli. The antibacterial activities of the complexes are stronger than those of the thiosemicarbazone itself.