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Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein

Authors :
Manoel Odorico de Moraes
Lisandra Juvêncio da Silva
Bruno C. Cavalcanti
Jacilene Silva
Hélio Vitoriano Nobre Júnior
Emmanuel Silva Marinho
Maria Erivanda França Rios
Cecília Rocha da Silva
Fátima Daiana Dias Barroso
João Batista de Andrade Neto
Amanda Dias Barbosa
Iri Sandro Pampolha Lima
Vitória Pessoa de Farias Cabral
Lívia Gurgel do Amaral Valente Sá
Francisca Bruna Stefany Aires do Nascimento
Source :
Journal of Medical Microbiology. 69:1221-1227
Publication Year :
2020
Publisher :
Microbiology Society, 2020.

Abstract

This study evaluated the effect of etomidate against biofilms of Candida spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: Candida albicans, Candida parapsilosis and Candida tropicalis. Growing biofilms were exposed to etomidate at 31.25–500 µg ml−1. Then, an ALS3 adhesive protein from C. albicans was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifungal activity against growing biofilms of resistant C. albicans, C. parapsilosis and C. tropicalis at all concentrations used in the study. The etomidate coupling analysis revealed three interactions with the residues of interest compared to hepta-threonine, which remained at the ALS3 site. In addition, etomidate decreased the expression of mannoproteins on the surface of C. albicans. These results revealed that etomidate inhibited the growth of biofilms.

Details

ISSN :
14735644 and 00222615
Volume :
69
Database :
OpenAIRE
Journal :
Journal of Medical Microbiology
Accession number :
edsair.doi...........31d260ba3f7cd310da3bb1d057324161
Full Text :
https://doi.org/10.1099/jmm.0.001241