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The role of cholesteryl ester transfer protein expression on endothelial cells: oxidative stress and vascular dysfunction
- Source :
- Free Radical Biology and Medicine. 108:S96
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- High-density lipoprotein cholesterol (HDL-C) levels are inversely correlated with development of atherosclerotic coronary heart disease and studies demonstrate that cholesteryl ester transfer protein (CETP) deficiency is associated with markedly increased HDL levels. HDL may exert atheroprotective activity by preventing endothelial dysfunction, a key step in the development of atherosclerosis. However, the CETP effects per se on endothelial function needs to be clarified. This study evaluates the role of CETP in the setting of early atherosclerosis and its contribution to endothelial dysfunction. In vitro, knockdown of Human Aortic Endothelial Cells (HAECs) with siCETP, prevented expression of adhesion molecules. Notably, eNOS activity was augmented in aortas of CETP-transgenic mice and correlated with decreased levels of inhibitory interaction with caveolin-1. Furthermore, the presence of CETP in aortas coincided with an increase in vascular production of reactive oxygen species evidenced by augmented oxidized to reduced glutathione ratio (GSSG/GSH), increased superoxide and hydrogen peroxide production and a marked decrease in endothelium-dependent vasorelaxation. Together, these findings suggest a role for CETP in promoting endothelial dysfunction and highlight the role in promoting oxidative stress.
- Subjects :
- chemistry.chemical_classification
Reactive oxygen species
medicine.medical_specialty
biology
Superoxide
Cell adhesion molecule
Glutathione
medicine.disease
medicine.disease_cause
biology.organism_classification
Biochemistry
carbohydrates (lipids)
chemistry.chemical_compound
Endocrinology
chemistry
Enos
Physiology (medical)
Internal medicine
Cholesterylester transfer protein
medicine
biology.protein
lipids (amino acids, peptides, and proteins)
Endothelial dysfunction
Oxidative stress
Subjects
Details
- ISSN :
- 08915849
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Free Radical Biology and Medicine
- Accession number :
- edsair.doi...........32d751253b5881eccc8cd1728bb435b7