qPercerus v1

Protocol for qPercerus SARS-CoV-2 Test (attached document is giving details). Final product will be a commercial kit that is consisting of four components (lysis buffer, primer/probe mix identification, primer/probe mix confirmation, qPCR master mix). The initial test for SARS-CoV-2 is the identification assay (FAM assay for ORF1ab with Cy5 assay as internal control) for patient screening. The confirmation assay (HEX assay for NSP10 with Cy5 assay as internal control) is used to re-evaluate the results.