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Exposure to environmental stress decreases the resistance of river microbial communities towards invasion by antimicrobial resistant bacteria

Authors :
Kenyum Bagra
Xavier Bellanger
Christophe Merlin
Gargi Singh
Thomas U. Berendonk
Uli Klümper
Publication Year :
2022
Publisher :
Cold Spring Harbor Laboratory, 2022.

Abstract

Environmental microbiomes are constantly exposed to invasion events through foreign, antibiotic resistant bacteria that were enriched in the anthropic sphere. However, the biotic and abiotic factors, as well as the natural barriers that determine the invasion success of these invader bacteria into the environmental microbiomes are poorly understood. A great example of such invasion events are river microbial communities constantly exposed to resistant bacteria originating from wastewater effluents. Here, we aim at gaining comprehensive insights into the key factors that determine their invasion success with a particular focus on the effects of environmental stressors, regularly co-released in wastewater effluents. Understanding invasion dynamics of resistant bacteria is crucial for limiting the environmental spread of antibiotic resistance. To achieve this, we grew natural microbial biofilms on glass slides in rivers for one month. The biofilms were then transferred to laboratory, recirculating flume systems and exposed to a single pulse of a model resistant invader bacterium (E. coli) either in presence or absence of stress induced by Cu2+. The invasion dynamics ofE. coliinto the biofilms were then monitored for 14 days. Despite an initially successful introduction ofE. coliinto the biofilms, independent of the imposed stress, over time the invader perished in absence of stress. However, under stress c the invading strain successfully established and proliferated in the biofilms. Noteworthy, the increased establishment success of the invader coincided with a loss in microbial community diversity under stress conditions, likely due to additional niche space becoming available for the invader.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........343858a49340a9c7fce3e1bac0ade044
Full Text :
https://doi.org/10.1101/2022.11.19.517188