Membrane fusion. Two possible mechanisms underlying a decrease in the fusion energy barrier in the presence of fusion proteins

Here we explored a contribution of fusion proteins to stalk formation, the first stage of membrane fusion, and considered two likely mechanisms, by which these proteins could influence the membrane transformation. One mechanism represents the induction of spontaneous membrane curvature, while another is membrane disturbance by a force generated by attached proteins. The energy barrier arising due to the deformation of approaching membranes and hydration repulsion between them was calculated. In addition, a dependence of an energy barrier height on certain protein features, such as spontaneous curvature, was analyzed. It was found that if fusion proteins do not produce a force directly applied to fusing membranes, they negligibly affect the barrier height irrespective of a value of spontaneous protein curvature. Thus, the overall results provide evidence that if fusion proteins are unable to exert force, they cannot provide monolayer fusion of the membranes.