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Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells
- Source :
- The EMBO Journal. 31:3678-3690
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- The eukaryotic genome is replicated according to a specific spatio-temporal programme. However, little is known about both its molecular control and biological significance. Here, we identify mouse Rif1 as a key player in the regulation of DNA replication timing. We show that Rif1 deficiency in primary cells results in an unprecedented global alteration of the temporal order of replication. This effect takes place already in the first S-phase after Rif1 deletion and is neither accompanied by alterations in the transcriptional landscape nor by major changes in the biochemical identity of constitutive heterochromatin. In addition, Rif1 deficiency leads to both defective G1/S transition and chromatin re-organization after DNA replication. Together, these data offer a novel insight into the global regulation and biological significance of the replication-timing programme in mammalian cells.
- Subjects :
- Regulation of gene expression
Genetics
0303 health sciences
Replication timing
General Immunology and Microbiology
Heterochromatin
General Neuroscience
DNA replication
Cell cycle
Biology
General Biochemistry, Genetics and Molecular Biology
Chromatin
Cell biology
03 medical and health sciences
0302 clinical medicine
DNA Replication Timing
Origin recognition complex
Molecular Biology
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- ISSN :
- 02614189
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi...........34fbaf1c62f65c362ddef6120103e33f